Description
Description: This assay employs a two-site sandwich ELISA to quantitate ESPL1 in samples. An antibody specific for ESPL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ESPL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ESPL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ESPL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 or STAG2 in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21) by ESPL1, or separase, which initiates the final separation of sister chromatids.Human separin associated with the protein encoded by the pituitary tumor-transforming gene (PTTG1), the human securin homolog, until activation of the APC.it shares 28% identity with Saccharomyces cerevisiae Esp1 and 30% identity with Schizosaccharomyces pombe Cut1. There is no similarity in the N terminus